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HuT 102
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AF Gazdar
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Biosafety Level:
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2 [Cells Contain RETROVIRUS ]
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frozen
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See Propagation
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suspension, multicell aggregates
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Homo sapiens (human)
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lymphoblast
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Disease: lymphoma; mycosis fungoides
Cell Type: cutaneous T lymphocyte;
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HTLV-I (human T cell leukemia virus)
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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interleukin 2 (IL-2)
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CD4; Homo sapiens
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Amelogenin: X,Y
CSF1PO: 8,11
D13S317: 11,13
D16S539: 12
D5S818: 8,13
D7S820: 8,10
THO1: 7,8
TPOX: 6
vWA: 16,19
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26 years
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male
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Black
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.Maintain cell density between 2 X 10 exp5 and 8 X 10 exp5 viable cells/ml. Do not allow density to exceed 1 X 10 exp6 cells/ml. Background debris is often present.
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
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about 41 hrs
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
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1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346
22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013
23184: Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591
23271: Poiesz BJ, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256
23311: Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351
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